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Invivo Preclinical Your Questions Answered: Volume One

Also see Volumes Two and Three

Can I implant intracranial electrodes in neonatal mice without drilling into the skull and damaging the brain?

Most likely no. Without drilling into the skull there isn't a good way to secure the electrode.

What is difference in the acute guide and standard guide cannula?

Acute guides have a recessed top for direct injection with syringe which allows easier insertion, while the standard cannula is made to be used with an injector cannula.

How do I sterilize components onsite?

Acceptable sterilization methods:

  • Guides, osmotic pump and injector cannulas(peek/ss)- can be steam autoclaved(max temp. 250 ℉)
  • Single dummy cannulas & dust caps can be steam autoclaved(1 cycle only-max temp. 250 ℉) **may swell slightly due to water absorption** Recommended method-dry heat (max temp. 250 ℉) or ethylene oxide
  • Bilateral dummy cannulas- can be steam autoclaved(max temp. 250 ℉)
  • Osmotic pump guide cannulas-cannot be steam autoclaved. Recommended method is ethylene oxide
  • Backmounts- recommended ethylene oxide and dry heat. Can be steam autoclaved(1 cycle only-max temp 250 ℉)

How can you secure the optic fiber to the optic fiber housing?

You can use an epoxy which will give the Optic fiber housing a stronger bond or super glue which is a more affordable option.

How can I prevent my rats from taking off their dummy cannulas?

Dummy cannulas are removed due to the natural grooming patterns of rodents. Counter clockwise grooming unscrews the dummy cannula making it easier for the animal to remove. After performing the implantation surgery, applying a bitter or spicy taste to the dummy can deter the animal from playing with the cannula. Another option is to screw a nut onto the guide to increase resistance.

Can you make a double guide and its mating components in MRI compatible material such as PEEK?

As long as there are no dimensional limitations we can handle most requests for customization.

Do you sell fiber optics?

P1 Technologies is proud to offer mating components used when doing optogenetics, but we do not currently carry fibers. We recommend contacting Thor Labs for your fiber needs.